Coding

Part:BBa_K3425037:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-19)


TEV protease for Type IIS standard (RFC1000)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 489
    Illegal NgoMIV site found at 795
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is codon optimised for E. coli and B. subtilis
This part is designed for the Type IIS iGEM standard assembly and therefore the coding sequence lacks the start codon. In Type IIS standard assembly, the ATG start codon is included in the coding-sequence prefix, as shown in the template CDS part (BBa_K3425019). You can also find useful design templates and more information about how to use this standard in the Type IIS guidebook of Uppsala 2020 team.

Source

Synthesized.

References